visual field mapping stimuli Search Results


g proteins mitogen activated protein kinase kinase 3 rac1 gtpase cycle rho guanine nucleotide exchange factor 4 guidance cues  (Cytoskeleton Inc)
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gene exp cxcl10 hs00171042 m1  (Thermo Fisher)
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anti p erk1 2  (Proteintech)
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sb203580  (Tocris)
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GDF-5 treatment increased the protein expression levels of TNMD via phosphorylation of <t>p38</t> in MSCs from murine compact bone. (A) MSCs from murine compact bone were treated with 100 ng/ml GDF-5 for 0, 5, 10, 30 and 60 min, and 6 and 24 h. Phosphorylation of p38 was determined by western blotting. (B and C) MSCs from murine compact bone were pretreated with 10 µM <t>SB203580</t> (an inhibitor of p38) for 1 h, and subsequently treated with 100 ng/ml GDF-5 for 30 min. (B) Phosphorylation of p38 and (C) TNMD expression were determined by western blotting. MSCs, mesenchymal stem cells; GDF-5, growth differentiation factor-5; TNMD, tenomodulin; p-p38, phosphorylated-p38.
Sb203580, supplied by Tocris, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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golgi stop  (Becton Dickinson)
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GDF-5 treatment increased the protein expression levels of TNMD via phosphorylation of <t>p38</t> in MSCs from murine compact bone. (A) MSCs from murine compact bone were treated with 100 ng/ml GDF-5 for 0, 5, 10, 30 and 60 min, and 6 and 24 h. Phosphorylation of p38 was determined by western blotting. (B and C) MSCs from murine compact bone were pretreated with 10 µM <t>SB203580</t> (an inhibitor of p38) for 1 h, and subsequently treated with 100 ng/ml GDF-5 for 30 min. (B) Phosphorylation of p38 and (C) TNMD expression were determined by western blotting. MSCs, mesenchymal stem cells; GDF-5, growth differentiation factor-5; TNMD, tenomodulin; p-p38, phosphorylated-p38.
Golgi Stop, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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map-aa4 peptide  (ICN Pharmaceuticals)
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GDF-5 treatment increased the protein expression levels of TNMD via phosphorylation of <t>p38</t> in MSCs from murine compact bone. (A) MSCs from murine compact bone were treated with 100 ng/ml GDF-5 for 0, 5, 10, 30 and 60 min, and 6 and 24 h. Phosphorylation of p38 was determined by western blotting. (B and C) MSCs from murine compact bone were pretreated with 10 µM <t>SB203580</t> (an inhibitor of p38) for 1 h, and subsequently treated with 100 ng/ml GDF-5 for 30 min. (B) Phosphorylation of p38 and (C) TNMD expression were determined by western blotting. MSCs, mesenchymal stem cells; GDF-5, growth differentiation factor-5; TNMD, tenomodulin; p-p38, phosphorylated-p38.
Map Aa4 Peptide, supplied by ICN Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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v3 kit  (10X Genomics)
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GDF-5 treatment increased the protein expression levels of TNMD via phosphorylation of <t>p38</t> in MSCs from murine compact bone. (A) MSCs from murine compact bone were treated with 100 ng/ml GDF-5 for 0, 5, 10, 30 and 60 min, and 6 and 24 h. Phosphorylation of p38 was determined by western blotting. (B and C) MSCs from murine compact bone were pretreated with 10 µM <t>SB203580</t> (an inhibitor of p38) for 1 h, and subsequently treated with 100 ng/ml GDF-5 for 30 min. (B) Phosphorylation of p38 and (C) TNMD expression were determined by western blotting. MSCs, mesenchymal stem cells; GDF-5, growth differentiation factor-5; TNMD, tenomodulin; p-p38, phosphorylated-p38.
V3 Kit, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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visual field mapping stimulus in matlab  (MathWorks Inc)
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MathWorks Inc visual field mapping stimulus in matlab
GDF-5 treatment increased the protein expression levels of TNMD via phosphorylation of <t>p38</t> in MSCs from murine compact bone. (A) MSCs from murine compact bone were treated with 100 ng/ml GDF-5 for 0, 5, 10, 30 and 60 min, and 6 and 24 h. Phosphorylation of p38 was determined by western blotting. (B and C) MSCs from murine compact bone were pretreated with 10 µM <t>SB203580</t> (an inhibitor of p38) for 1 h, and subsequently treated with 100 ng/ml GDF-5 for 30 min. (B) Phosphorylation of p38 and (C) TNMD expression were determined by western blotting. MSCs, mesenchymal stem cells; GDF-5, growth differentiation factor-5; TNMD, tenomodulin; p-p38, phosphorylated-p38.
Visual Field Mapping Stimulus In Matlab, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit monoclonal anti erk1 2  (Boster Bio)
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Boster Bio rabbit monoclonal anti erk1 2
GDF-5 treatment increased the protein expression levels of TNMD via phosphorylation of <t>p38</t> in MSCs from murine compact bone. (A) MSCs from murine compact bone were treated with 100 ng/ml GDF-5 for 0, 5, 10, 30 and 60 min, and 6 and 24 h. Phosphorylation of p38 was determined by western blotting. (B and C) MSCs from murine compact bone were pretreated with 10 µM <t>SB203580</t> (an inhibitor of p38) for 1 h, and subsequently treated with 100 ng/ml GDF-5 for 30 min. (B) Phosphorylation of p38 and (C) TNMD expression were determined by western blotting. MSCs, mesenchymal stem cells; GDF-5, growth differentiation factor-5; TNMD, tenomodulin; p-p38, phosphorylated-p38.
Rabbit Monoclonal Anti Erk1 2, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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golgi/stop (cytofix/cytoperm kit  (Becton Dickinson)
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Becton Dickinson golgi/stop (cytofix/cytoperm kit
GDF-5 treatment increased the protein expression levels of TNMD via phosphorylation of <t>p38</t> in MSCs from murine compact bone. (A) MSCs from murine compact bone were treated with 100 ng/ml GDF-5 for 0, 5, 10, 30 and 60 min, and 6 and 24 h. Phosphorylation of p38 was determined by western blotting. (B and C) MSCs from murine compact bone were pretreated with 10 µM <t>SB203580</t> (an inhibitor of p38) for 1 h, and subsequently treated with 100 ng/ml GDF-5 for 30 min. (B) Phosphorylation of p38 and (C) TNMD expression were determined by western blotting. MSCs, mesenchymal stem cells; GDF-5, growth differentiation factor-5; TNMD, tenomodulin; p-p38, phosphorylated-p38.
Golgi/Stop (Cytofix/Cytoperm Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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erk 1  (Shanghai Korain Biotech Co Ltd)
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Shanghai Korain Biotech Co Ltd erk 1
GDF-5 treatment increased the protein expression levels of TNMD via phosphorylation of <t>p38</t> in MSCs from murine compact bone. (A) MSCs from murine compact bone were treated with 100 ng/ml GDF-5 for 0, 5, 10, 30 and 60 min, and 6 and 24 h. Phosphorylation of p38 was determined by western blotting. (B and C) MSCs from murine compact bone were pretreated with 10 µM <t>SB203580</t> (an inhibitor of p38) for 1 h, and subsequently treated with 100 ng/ml GDF-5 for 30 min. (B) Phosphorylation of p38 and (C) TNMD expression were determined by western blotting. MSCs, mesenchymal stem cells; GDF-5, growth differentiation factor-5; TNMD, tenomodulin; p-p38, phosphorylated-p38.
Erk 1, supplied by Shanghai Korain Biotech Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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erk 1 2  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc erk 1 2
GDF-5 treatment increased the protein expression levels of TNMD via phosphorylation of <t>p38</t> in MSCs from murine compact bone. (A) MSCs from murine compact bone were treated with 100 ng/ml GDF-5 for 0, 5, 10, 30 and 60 min, and 6 and 24 h. Phosphorylation of p38 was determined by western blotting. (B and C) MSCs from murine compact bone were pretreated with 10 µM <t>SB203580</t> (an inhibitor of p38) for 1 h, and subsequently treated with 100 ng/ml GDF-5 for 30 min. (B) Phosphorylation of p38 and (C) TNMD expression were determined by western blotting. MSCs, mesenchymal stem cells; GDF-5, growth differentiation factor-5; TNMD, tenomodulin; p-p38, phosphorylated-p38.
Erk 1 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


GDF-5 treatment increased the protein expression levels of TNMD via phosphorylation of p38 in MSCs from murine compact bone. (A) MSCs from murine compact bone were treated with 100 ng/ml GDF-5 for 0, 5, 10, 30 and 60 min, and 6 and 24 h. Phosphorylation of p38 was determined by western blotting. (B and C) MSCs from murine compact bone were pretreated with 10 µM SB203580 (an inhibitor of p38) for 1 h, and subsequently treated with 100 ng/ml GDF-5 for 30 min. (B) Phosphorylation of p38 and (C) TNMD expression were determined by western blotting. MSCs, mesenchymal stem cells; GDF-5, growth differentiation factor-5; TNMD, tenomodulin; p-p38, phosphorylated-p38.

Journal: Molecular Medicine Reports

Article Title: Growth differentiation factor-5 induces tenomodulin expression via phosphorylation of p38 and promotes viability of murine mesenchymal stem cells from compact bone

doi: 10.3892/mmr.2017.8325

Figure Lengend Snippet: GDF-5 treatment increased the protein expression levels of TNMD via phosphorylation of p38 in MSCs from murine compact bone. (A) MSCs from murine compact bone were treated with 100 ng/ml GDF-5 for 0, 5, 10, 30 and 60 min, and 6 and 24 h. Phosphorylation of p38 was determined by western blotting. (B and C) MSCs from murine compact bone were pretreated with 10 µM SB203580 (an inhibitor of p38) for 1 h, and subsequently treated with 100 ng/ml GDF-5 for 30 min. (B) Phosphorylation of p38 and (C) TNMD expression were determined by western blotting. MSCs, mesenchymal stem cells; GDF-5, growth differentiation factor-5; TNMD, tenomodulin; p-p38, phosphorylated-p38.

Article Snippet: Cells were synchronized and pre-stimulated with 10 μM SB203580 (Tocris Bioscience, Bristol, UK; an inhibitor of p38 MAPK) at 37°C for 1 h. The control group was treated with an equivalent volume of 0.1% dimethyl sulfoxide.

Techniques: Expressing, Phospho-proteomics, Western Blot